Figure 4.

Single-copy snRNA loci also colocalize with CBs in interphase HeLa cells. Nuclei were stained with DAPI (blue); CBs are displayed in red; and snRNA gene signals are in green. In A (RNU11) and B (RNU12), BAC clones corresponding to the appropriate locus were used to generate the FISH signals; CB immunofluorescence was performed, and cells were scored as described previously (Frey and Matera, 1995). In each panel, a typical colocalization event is shown; the full data set is presented in Table 1.