TABLE 1.
Molecular mass determinations of the full-length NC1 domain and the endostatin domain of both murine type XV and XVIII collagens by sedimentation equilibrium. Samples were run in PBS at 4°C.
Protein | <U> (cm3 g−1) | RPM | Calculated mass (kDa) | AUC mass (kDa) | Oligomeric state | Concentration (mg/ml) |
---|---|---|---|---|---|---|
XV-ES | 0.7326 | 20,000 | 20.0 | 17.4±0.1/16.6±0.1 | 0.87/0.83 | 0.5/1.0 |
XVIII-ES | 0.7253 | 20,000 | 20.7 | 18.5±0.1 | 0.89 | 0.5 |
XV-NC1 | 0.7378 | 12,000 | 28.9 | 90.1±0.7/104.1±1. | 3.11/3.6 | 0.27/0.55 |
XVIII-NC1 | 0.7299 | 12,000 | 38.0 | 97.6±0.3 | 2.56 | 0.5 |