TABLE 2.

Sequences of peptides expressed as fusion proteins with the mini-fibritin. First and last numbers in peptide naming indicate the number of residues included from the carboxyl-terminal part of COL1 and the amino-terminal part of NC1, respectively. Numbering is given for the NC1(54) peptide only, where residues −1 and 0 do not belong to the NC1 domain. Underlined sequences are potential triple helix. Italics are non-natural sequences at the amino termini where GS were a part of the thrombin cleavage site (in all constructs) and P was added artificially for the continuous triple helix in constructs (66)COL1-NC1(69) and (66)COL1-NC1(136). The NC1(54) peptide contains only a single non-natural amino acid, G(−1) instead of A. Highlighted with gray are trypsin resistant region containing the trimerization domain.