Figure 2. Expression of the Fur regulon in Hpx− mutants.
(A) Aerobic defined medium. (B) Aerobic LB medium. WT: wild-type for Hpx. DP: Dipyridyl added. The strains were MS100 (Hpx+ iucC::lacZ), MS102 (Hpx− iucC::lacZ), and SMV59 (Hpx− iucC::lacZ furNI). The results represent the averages of three independent experiments. C) Levels of fur RNA transcript (top gel) and 5S RNA (bottom gel). Lane 1: MG1655 (WT, Hpx+); lane 2: SMV55 (Hpx+ furNI); lane 3: KCI402 (Hpx+ fur::kan); lane 4: LC106 (Hpx−).