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. 2011 Mar 4;6(3):e17687. doi: 10.1371/journal.pone.0017687

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Song et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The mRNA expression of Shh, Ptch, Smo, Gli1 and Gli2 in tumorsphere cells and adherent cells was analyzed by RT-PCR. The expression of GAPDH was used as a control. (B) The protein expression of SHH pathway target genes (Ptch and Gli1) was analyzed by Western blotting, β-actin was provided as a control. TCs = tumorsphere cells, ACs = adherent cells. (C) Quantitative real-time RT–PCR analyses of SHH pathway target genes (Ptch and Gli1) showed the same results of the semi-quantitative RT–PCR. Results were calculated as meant+s.d. values from triplicate measurements of three separate experiments. TCs = Tumorsphere cells, ACs = adherent cells.