Figure 6. Effect of SHH pathway blocking on tumor response to drugs in vivo.
(A) Subcutaneous tumors derived from 2×105 HGC-27 tumorsphere cells grown in nude mice 4 weeks after i.p. treatment with PBS or oxaliplatin in combination with 10 µM control tomatidine at the left rear flank around the tumor, or oxaliplatin in combination with 10µM cyclopamine at the right rear flank around the tumor, or oxaliplatin in combination with 10 ug/ml 5E1 at the right rear flank. Tom = tomatidine, Cyc = cyclopamine, Ox = oxaliplatin. TCs = tumorsphere cells. (B) Tumor size derived as in (A) after 20 days. Tom = tomatidine, Cyc = cyclopamine, Ox = oxaliplatin.