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. 2011 Mar 4;6(3):e17687. doi: 10.1371/journal.pone.0017687

Figure 7. SHH pathway maintained the gastric CSLCs characteristics of tumorsphere cells from primary tumor samples.

Figure 7

(A, B) Tumorspheres from gastric cancer specimen formed after 2 weeks later. Bars = 1000 µm. (C) Effect of 48 hours exposure of tumorsphere cells (white bars) and bulk cells (black bars) to 1000 µM chemotherapeutic drug. Ox = oxaliplatin, Mi = mitomysin. * = P<0.05. (D) Cell viability was analyzed on tumorsphere cells from gastric cancer specimen after exposure to cyclopamine or 5E1 with different drugs for 48 hours. Control group is PBS. Cyc = cyclopamine, Ox = oxaliplatin, Mi = mitomysin. (E) Subcutaneous tumors derived from 2×105 tumorsphere cells from gastric cancer specimen after i.p. treatment with PBS or oxaliplatin in combination with 10µM control tomatidine at the left rear flank around the tumor, or oxaliplatin in combination with 10µM cyclopamine at the right rear flank around the tumor. Tom = tomatidine, Cyc = cyclopamine, Ox = oxaliplatin. TCs = tumorsphere cells. (F) H&E staining analysis of human tumor and xenografts derived from tumorsphere cells. Bar = 100 µm.