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. 2011 Mar 4;6(3):e17709. doi: 10.1371/journal.pone.0017709

Figure 4. Factors contributing to rapid bactericidal activity of RS and manuka honey.

Figure 4

(A) RS honey; bacteria were incubated for 2 hours in various concentrations (v/v) of RS honey in incubation buffer (squares), with either catalase (asterisks), SPS (triangles) or both (diamonds) added, or in sugar solution (circles). (B) Manuka honey; B. subtilis was incubated for 2 hours in various concentrations of manuka honey in incubation buffer (squares), with successive addition of glyoxalase I (triangles, top up), SPS (triangles, top down), and titration to pH 7.0 (asterisks), or in sugar solution (circles).