Figure 2. Proliferation and activation of CD8+ T cells were inhibited in vivo in IL-6 treated mice.
C57BL/6 mice were treated with IL-6 plasmid and 2A as described in Figure 1 and further fed with drinking water containing 0.8 mg/mL of BrdU from day7 to day12. (A)At day12, the intrahepatic leukocytes were harvested and BrdU incorporation was analyzed by FACS. Percentage of BrdU+ CD8+ T cells in total CD8+ T cell and total number of BrdU+ CD8+ T cells in liver were shown. (B) The cytokine levels in the liver homogenate were compared. Representative data from 2 independent experiments with at least 3 mice per group is shown. *P<0.05 in comparison with control groups.