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. 2011 Mar 4;124(6):910–920. doi: 10.1242/jcs.073072

Fig. 3.

Fig. 3.

The starvation-induced decrease in levels of wild-type connexins is partially prevented by pretreatment with the lysosomal inhibitor chloroquine. (A) Immunoblot showing the levels of CX50 and β-tubulin in aliquots of total homogenates from HeLa-CX50 cells that were cultured in normal growth medium (lanes 1 and 2) or in HBSS for 6 hours (lanes 3 and 4) and left untreated (lanes 1 and 3) or treated with 100 μM chloroquine (CLQ) for 6.5 hours (lanes 2 and 4). (B) Levels of CX50 (expressed as a percentage of the values determined in cells grown in normal growth medium without CLQ) obtained from three independent experiments performed under the conditions described in A. (C) Immunoblot detection of CX43 in aliquots of total homogenates from NRK cells cultured in normal growth medium (lanes 1 and 2) or starved for 6 hours (lanes 3 and 4) that were left untreated (lanes 1 and 3) or treated with 100 μM chloroquine for 6.5 hours (lanes 2 and 4). (D) Levels of CX43 (expressed as a percentage of control, lane 1) detected in cells treated as described in C in three independent experiments.