Figure 3.

Rac1-DN causes morphological differentiation and actin bundle reorganization. Images of astrocytes infected with Ad-Rac1-DN and Ad-tetR-VP16 at high m.o.i. for 1 d (A–D) or 2 d (E–H), and stained with 9E10 and a secondary fluorescein-conjugated antibody to detect Rac1-DN (A, C and E), together with either α-GFAP and a TR-conjugated secondary antibody to identify the cells as astrocytes (B and F), or rhodamine–phalloidin (D and H). Bar (in H): A–C, E, and F, 27 μm; D, G, and H, 11 μm. Note that C and D are low- and high-magnification images, respectively, of the same cells. The cell on the left in C and D expresses Rac1-DN.