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. 1999 May;10(5):1665–1683. doi: 10.1091/mbc.10.5.1665

Figure 4.

Figure 4

Rac1* blocks and reverses bFGF-induced morphological changes. (A–F) Images of cells infected with Ad-Rac1* and left untreated (A–C) or treated with bFGF (D–F) for 2 d. Cells were stained with 9E10 and a fluorescein-conjugated secondary antibody to identify recombinant protein (A and D), together with α-GFAP and a TR-conjugated secondary antibody to identify the cells as astrocytes (B and E). Double exposures are shown in C and F. Note that uninfected cells in E have processes. The cells in A–C are from 3-wk-old cultures that exhibited some spontaneous morphological changes. (G–L) Images of cells that were pretreated with bFGF for 1 wk and then infected for 2 d with Ad-Rac1* (G–I) or Ad-GFP (J–L) and maintained in bFGF. G–I were stained as in A–F. J–L were stained only with the α-GFAP/TR secondary antibody. GFP is visible in the fluorescein channel (J). Double exposures are shown in I and L. Bar, 25 μm.