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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: J Immunol. 2011 Feb 9;186(6):3356–3363. doi: 10.4049/jimmunol.1000847

Fig. 5. IL-6 downregulation in bEnd.3 endothelial cells inhibits Th17 polarization.

Fig. 5

bEnd.3 or PAM212 cells were cultured overnight with 100 nM mouse IL-6 siRNA or the corresponding non-targeting control siRNA. Cells were then stimulated with or without the NOD2 ligand MDP for 3 hours. After extensive washing, LCs and T cells from transgenic mice were added along with cOVA323–339. Supernatants were harvested 48 hours later for cytokine content analysis. The graphs represent the mean of 3 independent experiments ± SD (***p<0.0001; **p<0.01; *p<0.05).