Skip to main content
. Author manuscript; available in PMC: 2012 Mar 31.
Published in final edited form as: Neuroscience. 2011 Jan 19;178:250–260. doi: 10.1016/j.neuroscience.2011.01.022

Figure 1.

Figure 1

TH down-regulates the expression of Islet2, Lim3 and axonin1 in the outer nuclear layer (ONL) in far peripheral regions of the postnatal chicken retina. Treated eyes received injections of 2 μg of TH at P0, P1, P2 and P4, whereas control eyes received injections of vehicle. Sections of the peripheral retina and CMZ were labeled with antibodies to Islet2 (a and b), Lim3 (d and e) and axonin1 (g and h). Double-ended arrows indicate the domain of the CMZ, and arrows indicate the domain of cells in the ONL that are positive for Islet2, axonin1 or Lim3. Areas indicated by rectangular boxes are enlarged 2-fold in the in-sets between panels a and b, and within panels e and h. The calibration bar (50 μm) in h applies to a, b, d, e, g and h. Histograms illustrate the means (± standard deviation) of the expression domains within the far peripheral ONL (c, f and i). The “expression domain” was measured as the linear, radial distance of retina (beginning at the CMZ) that contains cells with detectable levels of immunofluoresence. Significance of difference (*p<0.0001) was determined using a two-tailed student’s t-test. Abbreviations: ONL – outer nuclear layer, INL – inner nuclear layer, IPL – inner plexiform layer, CMZ – circumferential marginal zone.