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Triphenyltetrazolium chloride (TTC) staining can predict the zone of ablation after irreversible electroporation (IRE). TTC fixed liver tissue is shown 15 min (4a), 30 min (4b) and 1 h (4c) after IRE. The surrounding, viable liver tissue retains the red, triphenyltetrazolium chloride dye while dye washes out of the necrotic, ablated liver over time. The ablation zone is predictable at 15 min and becomes more apparent by 1 h. The zone of ablation is highlighted by the dotted white line
