Table 3.
Oligonucleotide primers used to amplify the 3 exons of AVPR2
| Primers Exon nbr | Size (bp) | Tm (°C) | Upstream primer | Downstream primer |
|---|---|---|---|---|
| AVPR2 cDNA 1 | 370 | 58 | 5'-GGAGTTCTGCGTGTCTGTCT-3' | 5'-TGAATCGTCAAACCCACTCT-3' |
| 2-1 | 416 | 59 | 5'-GCACAGCACCCTCTCTAACC-3 | 5'-GCCAGGATCATGTAGGAGGA-3' |
| 2-2 | 448 | 60 | 5'-GTGGCTCTGTTCCAAGTGCT-3' | 5'-CCAGACTGGCATGAATCTCC-3 |
| 2-3 | 441 | 60 | 5'-CGCACCTATGTCACCTGGAT-3' | 5'-GCCAGCAACATGAGTAGCAC-3 |
| 3 | 428 | 59 | 5'-TCCTGAACCCAACCTAGATCC-3 | 5'-ATACAGCTGGGGATGTGGAG-3 |
| 3'UTR | 436 | 60 | 5'-AGAATTGGCCAGAGCCTGT-3' | 5'-ACGTGACTGTGGGTGTGTGT-3' |
| AVPR2 cloning (BamHI/KpnI) | 5'-GCGCGGATCCACCATGCTCATGGCGTCC-3' | 5'-GCGCGGTACCCGATGAAGTGTCCTTGGC-3' |
Tm, annealing temperature; nbr, number; UTR, untranslated region