Table 1.

Effect of the Vpu_S52/56A mutant, β-TrCP1ΔF expression, or β-TrCP depletion by RNA interference on CD4 and CD317 surface levels, CD317 degradation and HIV-1 release enhancement by Vpu

	CD4	CD317		HIV-1 Release
	Downregulationa	Downregulationa	Degradationb	Enhancementc
Vpu	+	+	+	+
VpuS52/56A	-	-	-	-
Vpu + β-TrCP1 wt	+	+	+	+
Vpu + β-TrCP1ΔFbox	-	-	-	-
Vpu + β-TrCP1 KDd	+	+	+	+
Vpu + β-TrCP2 KDd	-	+	-	+
Vpu + β-TrCP1/2 KDd	-	+	n.a.	+

^aVpu-mediated downregulation of CD4 and CD317 from the surface of HIV-1-infected or transfected cells was quantified by flow cytometry (Figures. 1E-G; 2C, D; 4C; 8D)

^bVpu-mediated depletion of CD317 in HIV-1-infected or transfected cells was analyzed by quantitative immunoblotting (Figures. 1B; 2B) and/or immunofluorescence microscopy (Figures. 1C, D; 2E-F; 3A, B; 5B, C; 8B, C)

^cThe ability of Vpu to overcome the CD317-mediated HIV-1 release restriction was analyzed by quantification of infectious virus in culture supernatants (Figures. 1A; 2A; 3A, 5A; 8A)

^dKD: siRNA-mediated knockdown (verified by RT-PCR)

n.a.: not analyzed