Table 1.
HPLC measurements of uric acid, hypoxanthine and oxypurinol in urine, serum and tissues during the short-term administration of vehicle or allopurinol to DOCA-salt hypertensive rats.
| XO metabolite group (n) |
Uric acid vehicle (n=4) |
Uric acid allopurinol (n=4) |
Hypoxanthine vehicle (n=4) |
Hypoxanthine allopurinol (n=4) |
Oxypurinol vehicle (n=4) |
Oxypurinol allopurinol (n=4) |
|---|---|---|---|---|---|---|
| Sample (unit) | ||||||
| Urine day 0 (mg/day) | 2.508 ± 0.561 | 2.662 ± 0.383 | 0.017 ± 0.005 | 0.039 ± 0.005 | 0.017 ± 0.009 | 0.074 ± 0.026 |
| Urine day 18 (mg/day) | 1.687 ± 0.824 | 2.718 ± 0.165 | 0.029 ± 0.011 | 0.028 ± 0.006 | 0.255 ± 0.091 | 0.216 ± 0.118 |
| Urine day 31 (mg/day) | 1.776 ± 0.886 | 2.621 ± 0.597 | 0.016 ± 0.003 | 0.280 ± 0.096* | 0.170 ± 0.070 | 17.899 ± 4.357* |
| Serum (µg/g protein) | 154.0 ± 64.37 | 35.37 ± 6.903 | 0.0 | 48.54 ± 15.94* | 0.0 | 10.82 ± 8.724* |
| Aorta (µg/g protein) | 78.33 ± 26.34 | 15.24 ± 3.589* | 20.14 ± 10.16 | 67.75 ± 21.90* | ||
| Vena cava (µg/g protein) | 514.0 ± 173.7 | 100.8 ± 35.24* | 54.50 ± 17.06 | 155.2 ± 66.34* | ||
| Carotid artery (µg/g protein) | 91.68 ± 21.25 | 14.93 ± 2.556* | 30.06 ± 16.44 | 65.63 ± 16.70 | ||
| Jugular vein (µg/g protein) | 137.3 ± 10.11 | 27.78 ± 3.853* | 18.25 ± 4.369 | 51.16 ± 9.950* | ||
| Liver (µg/g protein) | 1039 ± 198.4 | 1064 ± 439.0 | 7.953 ± 6.760 | 37.52 ± 27.15 | ||
| Kidney (µg/g protein) | 3228 ± 131.7 | 3381 ± 1171 | 5.134 ± 2.800 | 514.9 ± 511.9* | ||
| Heart (µg/g protein) | 139.0 ± 32.80 | 71.28 ± 13.10 | 72.95 ± 6.872 | 368.2 ± 77.74* |
*
represents a statistically significant (p<0.05) difference between treatment groups.