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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mucosal Immunol. 2010 Nov 10;4(3):304–313. doi: 10.1038/mi.2010.75

Figure 5. Effect of macrophage-specific PPAR γ deletion on immune cell subsets in blood and spleen from mice with experimental IBD.

Figure 5

Blood (A–C) and spleen (D–F) from PPAR γ flfl; Lysozyme M-Cre+ and Lysozyme M-Cre− mice were immunophenotyped. Data were collected on day 7 of the dextran sodium sulfate (DSS) challenge and were analyzed with the FACS Diva software. Blood monocytes or spleen T cell subsets were selected by gating first on the viable cells based on FSC and SSC. Monocytes were then gated on F4/80+ CD11b+ and within this cell population, MCP-1 and MHC-II mean fluorescence intensity (MFI) were then examined. Splenocytes were gated on CD3+CD4+ and CD3+CD8+ or CD4+CD25+FOXP3+ to characterize the T cell subsets. Data are represented as mean ± standard error of groups of 10 mice. Points with an asterisk are significantly different (P<0.05).