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. Author manuscript; available in PMC: 2012 Jan 25.
Published in final edited form as: ACS Nano. 2010 Dec 16;5(1):399–407. doi: 10.1021/nn101839t

FIGURE 5.

FIGURE 5

Time courses of fluorescence intensity of Cy3-L11 labeled pre-complex (PRE-Lt, having tRNAfMet in P site and fMet-Arg-tRNAArg(Cy5) in the A site) close to a particle on a small silver particle coated surface. Cy3 (green) and Cy5 (red) fluorescence intensity traces of PRE-Lt complex close to silver particles (a) under 532 nm laser illumination show photobleaching of Cy5 at ~25 s. FRET efficiency distributions from fluctuating and non-fluctuating complexes are shown in b and c, respectively. (d) and (e) are dwell time distributions for high and low FRET states, respectively.