Figure 4.

Pol II occupancy at miRNA and siRNA loci. Pol II occupancy at several miRNA and siRNA loci was determined by ChIP using anti-RPB2 antibodies in Col and med20a. DNA present in the immunoprecipitates was quantified by real-time PCR relative to total input DNA. (A) ChIP performed with no antibodies as negative controls. (B) ChIP with anti-RPB2 antibodies. The results were reproduced in two biological replicates. Standard deviations were calculated from three technical repeats. AtSN1_A and soloLTR_A, regions A of these two loci as depicted in Figure 5B. AtSN1_B and soloLTR_B, regions B of these two loci as in Figure 5B.