Figure 6.

NF-κB activity was increased, and activities of several other transcription factors were decreased, in mouse lungs after challenge with IL-1F9. Mice were intratracheally challenged with 10 μg IL-1F9, and lungs were collected 2 hours and 6 hours later. Nuclear extracts prepared from lung tissue were used to assess nuclear factor activity, using multiplex assays in a Luminex 100 IS instrument. Data are reported as mean ± SEM of median fluorescence intensity (MFI) values, corrected for blank MFI values. (J) NF-κB activity was increased in lungs 2 hours after challenge with IL-1F9. No change was evident in NF-κB activity 6 hours after challenge with IL-1F9. (A–I, K) Transcription factors with decreased consensus DNA sequence binding activities, at either 2 hours or 6 hours after challenge with IL-1F9. Data represent mean ± SEM. *Significant differences from PBS-treated group (P < 0.05), n = 5 mice/group. AR, androgen receptor; HIF, hypoxia inducible factor; MEF2, myocyte enhancer factor–2; NF1, nuclear factor–1; NkX2-5, NK2 transcription factor–related locus–5; ETS/PEA, E-twenty six binding factor polyoma enhancer activator; ISRE, interferon-stimulated response element; MyoD, binding and myogenic differentiation factor.