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. 2010 Apr 15;44(2):238–243. doi: 10.1165/rcmb.2010-0007OC

Figure 2.

Figure 2.

Localization of KSGal6ST protein expression in normal mouse lung. (A) Two separate whole mouse lung extracts were run on gels in paired lanes (lanes 1–4) and probed with two different polyclonal antibodies recognizing KSGal6ST (Gel #1, probed with the rabbit antibody, lanes 1 and 2; Gel #2, probed with the sheep antibody, lanes 3 and 4). A single band occurred at approximately 45 kD. (B) Immunohistochemical localization of KSGal6ST protein in tissue sections of normal mouse lungs, using rabbit polyclonal antibody. Epithelial staining is particularly pronounced, but staining is also evident in some alveolar structural cells. Staining is blocked by preincubation with the KSGal6ST peptide used to create the polyclonal antibody. Results are shown from one of four experiments with identical findings. Original magnification, ×100. Original magnification, ×250 for insets.