Figure 3.

Effect of VEGFR and PKC inhibitors on VEGF induced tyrosine phosphorylation in mesothelioma cells. (a) Inhibition of VEGF-induced tyrosine phosphorylation in H2461 malignant mesothelioma cells by KRN633 and enzastaurin alone or in combination. Serum-starved mesothelioma H2461 and Met5A cells were pre-incubated with KRN633 1 µg/ml (4 hours) and/or enzastaurin 2.5 µM for 24 hours. Cells were treated with VEGF (20 ng/ml) for 20 minutes before collecting the lysates. Cell lysates were resolved by 7.5% SDS-PAGE and probed with mouse monoclonal p-Tyr antibody (Tyr 951). (b) VEGF-induced phosphorylation signaling status of VEGFR-2, PKC-β2 and AKT in the presence of KRN633 and/or enzaustarin in H2461 or Met5A cell lines.