Fig. 3.

Genes in the odd skipped cluster are targets of Hh signaling in the dorsal ectoderm of Drosophila. (A) DamCi^Act (orange vertical bars) and DamCi^Rep (blue vertical bars) signal at drumstick (drm), sister of odd and bowl (sob) and odd skipped (odd). Red boxes indicate Ci motif clusters matching the motif YGSGDGGNC (see Table S1 in the supplementary material). (B-E) Wild-type expression patterns of ptc (B), drm (C), sob (D) and odd (E). Expression of ptc coincides with the anterior margin of the tracheal pit (white line in B). Stripe of drm expression lies immediately posterior to hh-lacZ (brown staining in C). (F-I) Expression of drm (G), sob (H), and odd (I) expands posteriorly by two to three cell diameters in daGal4>UAS Ci^Act. ptc expression expands similarly (F; arrow) and is activated in more cells of each parasegment. (J) Diagram of Hh target gene activation in the dorsal ectoderm. ptc expression (tan) on the anterior and posterior flanks of each hh stripe (blue) expands with ubiquitous Ci^Act (right; red stripes), but expression of Hh targets odd, drm and sob (brown), which in wild type (WT, left) is posterior to the dorsal portion of each hh stripe, expands only on the dorsal-posterior flank.