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. 2010 Nov 15;137(22):3899–3910. doi: 10.1242/dev.050021

Fig. 1.

Fig. 1.

LYVE1-positive macrophages are intimately associated with lymphatic vessels but do not express PROX1. Confocal z-stack images of 60 μm sections demonstrating the localisation of LYVE1-positive, GFP-positive macrophages in close association with, and apparently integrated within (arrowheads), the jugular lymph sac (JLS; A-C) and dermal lymphatic vessel (DLV; D-G) endothelium of E14.5 c-fmsEGFP embryos. G illustrates a 90° rotation of F in order to view the lymphatic vessel in cross-section. Blue and white arrowheads denote two macrophages that appear integrated within this DLV, both of which express GFP, LYVE1 and F4/80. LYVE1-positive macrophages that are apparently integrated within the JLS or DLV endothelium do not express PROX1 (H-O). White circles in K and O illustrate the location of macrophages (arrows) in J and N. K and O illustrate single-channel PROX1 images of J and N, respectively. Data are representative of at least four independent experiments. Scale bars: 150 μm in A; 100 μm in D,H,L; 50 μm in B,E,I,M; 25 μm in C,F,J,K,N,O.