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. 2011 Mar 1;25(5):445–459. doi: 10.1101/gad.2014311

Figure 1.

Figure 1

SSA inhibits U2 snRNP-containing A complex assembly. (A) In vitro splicing assays using AdML RNA in the presence of the indicated concentrations of SSA or its carrier (methanol), analyzed by denaturing polyacrylamide gel electrophoresis. Pre-mRNA, splicing intermediates, and products are indicated. (B) Spliceosome assembly assays corresponding to in vitro splicing mixes as in A in the presence of heparin (5 μg/μL), analyzed by native agarose–polyacrylamide gel electrophoresis. The positions of heterogeneous nuclear RNP (H) and spliceosomal A, B, and C complexes are shown. (C) Spliceosome assembly assays as in B using AdML 3′ RNA containing the 3′ 40 nt of AdML intron 1 and exon 2. The positions of the A3′ and H complexes are indicated.