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. 2011 Mar 3;2011:187624. doi: 10.1155/2011/187624

Table 1.

Summary of the FRAP results obtained for recovery rates and dissociation constants (K off) extracted from Figures 25 for H2B, H4, and MacroH2A. The analysis was subdivided into 3 categories: control cells (in italic), silenced cells (bold), and comparison between silenced cells and control cells (italic-bold). In addition to the 2 parameters directly extracted from the results, implication of a third parameter was obvious to explain the observed recovery rates, the number of bleached histone molecules disassembled.

H2B H4 MacroH2A
Relative recovery rate at 1h30 in control cells faster (37%) slower, but variable (24%) slower (27%)
Relative Koff in control cells medium faster slower
Implication for the number of bleached histone molecules disassembled in control cells higher number compared to H4 lower number compared to macroH2A compatible with same number for H2B

Recovery rate in silenced nucleolin cells at 1h30 faster slower, but variable faster
Koff in silenced nucleolin cells compared to control cells slower slower in any case faster
Implication for the number of bleached histone molecules disassembled in silenced cells higher number compared to H4 smaller number compared to macroH2A higher number compared to H2B

Recovery rate in silenced nucleolin cells compared to control cells faster identical  when splitted into a fast and a slow population faster
Koff in silenced nucleolin cells compared to control cells slower slower in any case identical
Implication for the number of bleached histone molecules disassembled in silenced cells higher number compared to control higher number compared to control higher number compared to control