Fig. 2.

Epitope-arrays performed with the phage libraries obtained after three rounds of panning with mAb EP498Y (A) and mAb 8015 (B). The arrays, consisting of 72 oligonucleotides spanning the nucleotide sequence of the extracellular portion of CD22, were probed with fluorescently labelled PCR products from the round three phage selection outputs. Hybridisation controls UP and RP are positive controls for labelling and library amplification, which are complementary to BlaP sequences upstream and downstream the insertion site. T7 and surrounders bars are mismatched UP controls (90, 80 and 60% of mismatch) used to monitor washing steps and hybridisation specificity. (C) Representation of regions 1, 2 and 3 on the primary amino acid sequence of human CD22.