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Isolation of a hybrid protein, comprising beta-lactamase with a CD22 fragment insertion, for conducting the mutational analysis. (A) Phage ELISA of different hybrid proteins performed on 200 ng of coated mAb 8015 with supernatants of isolated phage culture. Nitrocefin (100 µM) was used as reporter substrate. (B) Multiple sequence alignment of the 14 candidates selected in (A).
