Fig. 5.

Summary of the binding of CD22 fragments (V234–G435), each containing single-alanine substitutions, to CAT-8015 IgG1, normalised to the binding of the wild-type CD22 fragment. (A) Statistical analysis of the residual binding data according to the ANOVA method allowed grouping into four distinct groups: coloured red, orange, yellow and green, based on the loss of binding signal as a result of alanine substitution (red, 0–20% residual binding; orange, 20–50% residual binding; yellow, 50–80% residual binding; green, 80–100% residual binding). Results are presented as box-and-whisker plots, with the bold line representing the median value and boundaries of the box representing the 25th and 75th percentile. The bars represent the data extremes. (B) Representation of the same residues, with the same colouring scheme, on the amino acid sequence of CD22 fragment (V234–G435).