Figure 4.

Looping profile of EBV-LCL cell line derived from a Beckwith patient with pUPD. (A) Locus as in Figure 3. (B) Looping profile of pUPD compared with EBV control when the CTCF DS is used as an anchor, showing strong interactions with elements 5′ of the ICR such as the CCD and the CTCF AD. No interaction is seen with the enhancer or ICR. (C). Using the ICR anchor, no difference is seen in the two cell lines for the ICR–CTCF AD interaction, whereas the ICR–CTCF DS interaction is absent in the pUPD cell line. The paternally methylated ICR does not interact with the CTCF DS, and the CTCF DS interacts with the CTCF AD on the paternal allele.