Figure 1. pAgs induce heightened airway allergic responses compared with sAgs.

Allergen-sensitized mice were challenged with HDM or RW in sAg or pAg form, PBS, or unconjugated particles (BLK P; i.e., blank particles). (A–C) At 48 hours after challenge, (A) AHR (quantified by average resistance measurements; Ave R_T), (B) BAL eosinophil influx, and (C) lung histology were analyzed (n = 5). *P < 0.05, pAg versus sAg; ^§P < 0.01, sAg versus PBS; ^#P < 0.01, pAg versus unconjugated particles. (D) H&E-stained lung tissues. B, small bronchioles; V, vasculature. Insets show larger views of inflammation sites. Scale bars: 25 μm; 200 μm (insets). Each photomicrograph is representative of 4 mice.