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. 2011 Feb 1;121(3):941–955. doi: 10.1172/JCI43584

Figure 3. Differential cytokine and chemokine responses of BMMCs after exposure to sAgs or pAgs.

Figure 3

TNP-specific IgE–sensitized BMMCs were challenged with 1 μg sAg (OVA/TNP) or pAg (particles coated with OVA/TNP). (A) At 1 hour after allergen exposure, MC degranulation was assessed by β-hexosaminidase release (n = 3). §P < 0.01 versus vehicle. (B) De novo synthesis of proinflammatory mediators. At 1 hour after exposure, mRNA expression levels of IL-4, IL-5, IL-13, TNF-α, and eotaxin-1 in BMMCs were determined by real-time PCR (n = 4). Untreated BMMCs, unsensitized BMMCs challenged by sAgs or pAgs, and sensitized BMMCs without challenge or exposed to blank beads served as controls. §P < 0.01 versus controls; *P < 0.01 versus sAg. (C) Anti-phosphotyrosine Western blot of proteins from IgE-sensitized BMMCs following exposure to sAgs or pAgs. In all cases (arrows) except a 52-kDa band (arrowhead), phosphorylation evoked by pAgs appeared appreciably more sustained than that by sAgs. Blot is representative of 4 separate experiments. The intensity of phosphorylation of prominent proteins at various time points was also assessed using densitometry, using actin as the internal quantitative control. *P < 0.01 versus sAg.