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. 2011 Feb 14;121(3):930–940. doi: 10.1172/JCI43871

Figure 5. Specific modulation of complex I activity by mitochondrial MEF2D.

Figure 5

(A) Requirement of mitochondrial MEF2D for complex I activity. Overexpression of Mt2Ddn reduced mitochondrial complex I activity, revealed by BN-PAGE and in-gel activity staining. Coomassie blue staining showed specific reduction of complex I protein level after overexpression of Mt2Ddn (n = 3; **P < 0.01). Control indicates the control vector group. (B) Overexpression of ND6 rescued complex I activity reduced by Mt2Ddn. Control indicates the control vector group. (C) Measurement of various complex activities. Quantitative analysis of mitochondrial complex activities showed specific reduction of complex I activity by Mt2Ddn in SN4741 cells (n = 4; **P < 0.01). Control indicates the control vector group. (D) Effect of overexpression of Mt2D or Mt2Ddn on mitochondrial function. Mt2Ddn reduced cellular ATP level and elevated H2O2 production in SN4741 cells (n = 4; *P < 0.05, **P < 0.01). Δym, loss of mitochondrial membrane potential. Control indicates the control vector group.