Figure 3. Limited growth and differentiation potential of hiPSC-derived cells.
(A) Representative bioluminescence images of immunocompromised SCID beige mice implanted with 106 hESCs or 106 hiPSCs stably expressing firefly luciferase reporter construct. (B) Quantitative analysis of bioluminescence imaging data shows slower teratoma growth kinetics of hiPSC-derived teratomas (mean ± SEM for n = 10). sr, steradian. (C) Immunostaining for cardiac troponin T (cTnT) of hESC- and hiPSC-derived cardiomyocytes (original magnification, ×20). (D) Assessment of the percentage of cell aggregates containing beating cardiomyocytes after 14 days shows substantial variation in the yield of hiPSC-CMs, while the yield of hESC-CMs is stable (mean ± SEM for n = 8). (E) Immunostaining for CD31 (left) and CD144 (top right) EC markers and robust LDL uptake (bottom right) for hESC-derived ECs after 14 days of differentiation before (bottom left) and after (top left and top right) FACS enrichment (original magnification, ×20). (F) Cell proliferation and CD31 expression over 2–3 weeks after isolation of EC populations show limited stability and proliferative capacity of hiPSC-ECs in comparison with hESC-ECs (mean ± SD for n = 4).