Figure 6. Raf1^L613V mutant increases Mek and Erk activation in cardiomyocytes.

Cardiomyocytes prepared from neonatal WT and L613V/+ mice were starved for 24 hours and then stimulated for the indicated number of minutes with 1 μg/ml Ang II (A), 10 ng/ml IL-6 (B), and 100 ng/ml heregulin-β1 (C). Cell lysates (15 μg protein) were immunoblotted with the indicated antibodies. Quantification of blots is also shown. 1 of 2 experiments with comparable results is shown.