Figure 1.

Integrated systems biology strategy to study cellulosic bioconversion. Cultures metabolizing different biomass substrates were examined for (A) growth and biomass consumption rates (Figure 2A–C), (B) fermentation production rates and yields (Figure 2D–F), and (C) ability of the microbe to adhere to cellulosic substrates (Figure 2G–I). (D) Supernatant and cellular protein samples were taken for reductive dimethylation (ReDi) proteomics and analyzed for enzyme secretion (Figure 4), abundances of cellulolytic enzymes (Figure 5), and proteome-wide changes (Figure 6). (E) These data were integrated to identify key enzymes for each step in biomass deconstruction and fermentation (Table I, Figure 7).