Figure 4.

The C. phytofermentans secretome. (A) Proteins with high-scoring N-terminal signal peptides have a greater probability of being in culture supernatants. Fraction of proteins in proteome at each SignalP-NN D value observed in the supernatants of hemicellulose or cellulose cultures. Data were fit to a piecewise linear regression with the leftmost regression to a horizontal line. Consensus sequences of (B) type I and (C) type II lipoprotein N-terminal signal peptides for proteins found in the supernatant of cellulose cultures. (D) Functional categories of proteins in culture supernatants: rust, flagellum; turquoise, cell wall/surface; gray, proteases; orange, transport; green, CAZy; purple, other; gold, unknown. (E) Transmission electron micrograph of a C. phytofermentans cell cross section showing the cell membrane, cell wall, and surface layer.