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. 2011 Jan 18;7:461. doi: 10.1038/msb.2010.116

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Copyright © 2011, EMBO and Macmillan Publishers Limited

This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.

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Carbohydrate-active enzyme (CAZy) expression and activities in glucose, hemicellulose, and cellulose cultures. (A) Secreted and cellular cellulolytic enzyme activities. Protein lysates from cultures grown on glucose (purple), hemicellulose (turquoise), or cellulose (orange) prepared from the cellular fraction (C), supernatant (S), or whole-culture lystates (L). Proteins were incubated with hemicellulose (hemicellulase assay) or carboxymethylcellulose substrate (cellulase assay), reducing sugars were assayed using dinitrosalicyclic acid, and were normalized to protein concentration. (B) CAZy expression changes (MS1 peak area ratio, MPA ratio) on hemicellulose and cellulose versus glucose showing differentially expressed proteins (P<0.01) on hemicellulose (turquoise), cellulose (orange), or both (green). Symbols show cellular proteins (circles) and supernatant proteins (triangles). (C–E) Shifts in the relative abundances of CAZy proteins in glucose (C) hemicellulose (D), and cellulose (E) treatments by Absolute Protein EXpression (APEX) show acclimation to different carbon sources. Fraction of proteome comprised of CAZy proteins in each treatment is shown.