Figure 4.

BAG3 associates with aggresomes in vivo. (A) Spinal cord sections of mice overexpressing SOD^WT and SOD^G85R (disease end-stage) were immunostained for BAG3 and SOD1. Arrows indicate SOD1- and BAG3-positive aggregates in the neuropil. Scale bar, 20 μm. (B) Localization analysis of BAG3 and SOD^G85R in surviving spinal cord motor neurons from SOD^G85R mice at the time of disease symptom onset. Arrows indicate perinuclear aggresomes containing BAG3 and SOD^G85R. Scale bar, 10 μm. Magnific., magnification; SOD, superoxide dismutase; SOD^WT, wild-type SOD.