Figure 1.

Effect of Am80 on intracerebral hemorrhage (ICH)-induced neuronal damage. (A) A representative image of a NeuN-immunostained coronal section obtained 3 days after collagenase injection. The central region and the peripheral region for cell counts were denoted by rectangles with and without an asterisk, respectively. Scale bar=1 mm. (B–E) Representative images of the area corresponding to the center of hematoma in NeuN-immunostained coronal sections obtained 3 days after induction of ICH. Mice received intrastriatal injection of saline (Sal; B) or collagenase (Col; C–E). Oral administration of vehicle (0.5% carboxymethyl cellulose (CMC); B and C) or Am80 at indicated doses (D and E) was performed once daily for 4 days starting from the day before induction of ICH. Scale bar=50 μm. (F and G) The number of NeuN-positive cells in the central (F) and the peripheral (G) regions of hematoma was quantified. n=6 to 15 for each condition. ^***P<0.001 versus sham group; ^###P<0.001 versus CMC group (analysis of variance results: for E, F_3,36=191.95, P<0.0001; for F, F_3,36=463.92, P<0.0001).