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. 2010 Jun 30;31(1):328–338. doi: 10.1038/jcbfm.2010.97

Figure 1.

Figure 1

Light microscopic findings in the second to fifth cortical layers of Face-A. (A) Coronal section of Face-A and -B 72 hours after ischemia (periodic acid-fuchsin-Schiff, PAS). The four cortical columns counted, each 0.25 mm in width, are indicated. ‘1/2' indicates the point medial between rhinal (RF) and interhemispheric (IF) fissures along the cortical surface. (B–E) Hematoxylin-eosin (HE) staining; bars=30.4 μm. (B) At 0.5 hour after ischemia, degenerated (arrows) and dead (arrowheads) neurons are seen. (C) At 5 hours after ischemia, degenerated (black arrows) and dead (arrowhead) neurons are seen along with obstructed microvessels showing stasis and surrounded by swollen end-feet (blue arrows). (D) By 12 hours after ischemia, flocks of foamy necrotic neuropils (black arrows) are noted among necrotic neurons. Blue arrows indicate patent microvessels. (E) At 24 hours after ischemia, dead neurons are scattered in diffusely spongy necrotic tissue. Almost all microvessels are patent (blue arrows). (F) At 3 hours after ischemia: carbon-black-suspension perfused (CBSP) and HE, bars=30.4 μm. Carbon-black-suspension-perfused (blue arrows) and not-perfused (black arrows) microvessels are seen. (G) At 5 hours after ischemia, signs of stasis (arrows) and obstructed microvessels (arrowhead) are observed (Toluidine blue), bars=12.2 μm.