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. 2010 Jun 30;31(1):339–350. doi: 10.1038/jcbfm.2010.98

Figure 4.

Figure 4

Striatal injection of lentivirus-expressing salt-induced kinase 1 (SIK1) small interference RNA (siRNA) increased infarct sizes in castrated mice supplemented with a protective dose of dihydrotestosterone (DHT) (0.5 mg). (A) Intrastriatal injection of lentivirus resulted in robust enhanced green fluorescence protein (eGFP) signal in the striatum at 2 weeks after injection, and eGFP signal was absent in the noninjected, contralateral side. (B) Lentivirus-delivered SIK1 siRNA significantly decreased striatal SIK1 mRNA expression in injected, ipsilateral sides (Ipsil) versus noninjected, contralateral sides (Contra) at 2 weeks after injection (n=5). *P<0.05. (C) In castrated mice implanted with the protective dose of DHT, intrastriatal injection of lentivirus-expressing SIK1 siRNA (n=9) increased striatal, cortical, and hemispheric infarct volumes compared with injection of lentivirus-expressing nonsense siRNA (LV-non, n=10). *P<0.05. Infarct volumes (DHT-supplemented castrates injected with LV-non versus DHT-supplemented castrates injected with siRNA): 12.1±1.7 versus 19.6±2.5 mm3 in the cortex, 8.5±0.6 versus 9.9±0.5 mm3 in the striatum, and 36.8±3.0 versus 55.0±6.2 mm3 in the hemisphere.