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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Neurosci Res. 2010 Oct 16;69(2):154–160. doi: 10.1016/j.neures.2010.10.003

Figure 2.

Figure 2

Effects of PSAT1 knockdown by RNAi on L-serine level. A) Successful knockdown of PSAT1 by siRNA in primary rat astrocytes. Primary rat astrocytes were transfected with siRNA against PSAT1 or control siRNA. Cells were harvested on day 7 and examined for PSAT1 protein expression by Western blot analysis by using anti-PSAT1 antibody. GAPDH expression level was examined for internal control. (-); no transfection. Bar graph shows PSAT1 expression level relative to GAPDH. B) Amino acids concentration in culture supernatants of primary rat astrocytes with PSAT1 siRNA. Astrocytes transfected with either PSAT1 siRNA or control siRNA was determined at 24 h after the last medium change (day 7 post-transfection) by a high-performance liquid chromatography (HPLC) system. Error bars represent standard deviation. NT; non-treated, Con; treated with control siRNA, and Exp; treated with PSAT1 siRNA. **p=0.00175, Student t-test. Data are representative of three independent experiments.