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. 2011 Jan 11;104(3):448–459. doi: 10.1038/sj.bjc.6606064

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Copyright © 2011 Cancer Research UK

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The CITCO induces apoptosis in BTSCs. The T98G, U87MG, DB29 and DB33 glioma cells were cultured in NBM with B27 and EGF+bFGF. The gliospheres generated in 5 days were cultured in fresh NBM with B27 and EGF+bFGF in the presence of 0, 2.5, 5 or 10 μM CITCO. After 48 h, the gliospheres were dissociated (BTSCs), stained with Annexin V-FITC along with propidium iodide and analysed by flow cytometry. The figure is representative of three independent experiments. The histogram shows the mean Annexin V-positive BTSCs (±s.d.) of three experiments. ^*P<0.05, ^**P<0.001, ^***P<0.001.