Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jan 4;104(3):505–513. doi: 10.1038/sj.bjc.6606070

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2011 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Immunohistochemical analysis of the SV40 large T antigen after sorting of cells in contact cocultures. PC-3 human prostate cancer cells (labelled with DiOC₁₈(3)) were mixed and cocultured with DiIC₁₈(3)-labeled human osteoblasts expressing SV40 (hFOB1.19) for 48 h. Cocultured prostate cancer cells (DiOC₁₈(3)⁺/DiIC₁₈(3)⁻) and hFOB1.19 cells (DiOC₁₈(3)⁻/DiIC₁₈(3)⁺) were then separated using flow cytometry. One thousand cells from each sorted cell population were cultured overnight on glass slides and were then immunohistochemically stained for the SV40 large T antigen. Positive staining of the SV40 large T antigen was observed in the nuclei of hFOB1.19 cells (DiOC₁₈(3)⁻/DiIC₁₈(3)⁺) but not in PC-3 cells (DiOC₁₈(3)⁺/DiIC₁₈(3)⁻). Scale =100 μm.