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. 2011 Jan 4;104(3):505–513. doi: 10.1038/sj.bjc.6606070

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Copyright © 2011 Cancer Research UK

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Osteoclastogenic activity of PC-3 cells cocultured with hFOB1.19 cells. The effect of addition of a cell lysate from PC-3 cells, grown under contact or bilayer coculture conditions, on osteoclastogenesis of bone marrow cells in an in vitro osteoclastogenesis assay, was determined by microscopic analysis of the number of tartrate-resistant acid phosphatase (TRAP) cells. The number of TRAP-positive cells in more than five microscopic fields was counted ( × 200 magnification). Osteoclastogenesis was significantly enhanced by the cell lysate of PC-3 cells grown under contact coculture conditions compared with osteoclastogenesis with a lysate of cells grown under bilayer coculture conditions (P<0.005). Data are representative of two separate experiments.