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. 2011 Jan 4;104(3):505–513. doi: 10.1038/sj.bjc.6606070

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Copyright © 2011 Cancer Research UK

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Effect of the blockade of N-cadherin- and cadherin-11-dependent cell adhesion between PC-3 and hFOB1.19 cells. (A) Confirmation of N-cadherin and cadherin-11 expression in both PC-3 cells and hFOB1.19 cells using RT–PCR. (B) Effects of N-cadherin or cadherin-11 neutralising antibodies on osteoclastogenesis-related gene expression in PC-3 cells under contact coculture conditions. The hFOB1.19 cells were pretreated with N-cadherin- or cadherin-11-neutralising antibodies for 30 min at 37°C, and then contact coculture with PC-3 cells was initiated. After 48 h, RNA was extracted from sorted PC-3 cells and was used as a template for RT–PCR to analyze the expression of the indicated osteoclastogenic genes. Signal intensities of each band were quantified by using NIHimage 1.62 software and normalised by that of β-actin. Data are representative of two separate experiments. The cadherin-11 neutralising antibody inhibited the upregulation of COX-2 and C3 mRNA, whereas the N-cadherin neutralising antibody further induced the upregulation of COX-2 mRNA.