Table 1. Polyclonality of BRAF mutations in primary melanoma as revealed by single-cell PCR and sequencing.
| Sample no. | Age (years) | Sex | Type of melanoma | No. of cells with heterozygous BRAF mutationsa | No. of cells with homozygous BRAF mutationsa | No. of cells with wild-type BRAF | Total no. of cells |
|---|---|---|---|---|---|---|---|
| PM-1 | — | F | Acral | 7 (V600E)+2 (K601R) | 2 (V600E) | 45 | 56 |
| PM-2 | 21 | F | NCSD | 14 (V600E) | 3 (V600E) | 31 | 48 |
| PM-3 | 53 | F | NCSD | 4 (V600E)+3 (V600M) | 3 (V600E)+2(V600-K601E) | 40 | 52 |
| PM-4 | 85 | F | Acral | 8 (V600E) | 1 (V600E) | 41 | 50 |
| PM-5 | 47 | F | Acral | 0 | 0 | 50 | 50 |
| MMG1 | — | — | Acral | 29 (V600E) | 1 (V600E) | 10 | 40 |
Abbreviations: Acral=acral melanoma; MMG1=a cell line established from primary acral melanoma; NCSD=melanoma on non-chronic sun-damaged skin; PM=primary melanoma.
a
V600E, T1799A; K601R, A1802G; V600M, G1798A; V600-K601E ,TGA1799-1801del.